2011 BD Accuri C6 Flow Cytometry

BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD Accuri C6 Flow Cytometer Specifications

Laser Excitation 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life)

Laser Profile 10 x 75 µm

Laser Power 14.7 mW 640 nm

Diode Red Laser 20 mW 488 nm

Solid State Blue Laser

Scatter Detection

Forward (0 degrees, +/-13)

Side (90 degrees, +/-13)

Emission Detection 4 colors, standard optical filters

• FL1 533/30 nm (e.g. FITC/GFP)

• FL2 585/40 nm (e.g. PE/PI)

• FL3 > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7)

• FL4 675/25 nm (e.g. APC)

Optical Alignment

Fixed alignment Flow Cell 200 µm ID quartz capillary

Minimum Detectable Particle Size 0.5 µm

Minimum Sample Volume 300 µL (in 12 x 75 mm tube)

Pre-Set Flow Rates and Core Sizes

Slow: 14 µL/min, 10 µm core

Medium: 35 µL/min, 16 µm core

Fast: 66 µL/min, 22 µm core

Custom Sample Flow Rates 10 – 100 µL/min

Custom Core Diameter 5 – 40 µm

Recommended Sheath Fluid 0.22 µm filtered, deionized (DI) water

Maximum Events Per Sample 1 million events per well

Florescence Sensitivity, MESF* FITC < 150; PE < 100

Scatter Resolution

Resolves human peripheral blood lymphocytes, monocytes, and granulocytes

Fluorescence Linearity 2 ± 0.05% for chick erythrocyte nuclei (CEN)

Fluorescence Precision ≤ 3%

CV for CEN Data Acquisition Rate 10,000 events/second, maximum

Power Requirements 100 - 240 VAC, 50/60 Hz

Typical Power Consumption 154 VA

Heat Output 240 Btu/hour maximum output

Operating Ranges 15 - 30° C; < 80% relative humidity Instrument

Size 11”H x 14.75”W x 16.5”D (27.9 x 37.5 x 41.9 cm)

Foot Print with Fluid Bottles 11”H x 21.5”W x 16.5”D (27.9 x 54.6 x 41.9 cm)

Weight 30 lbs (13.6 kg)

Fluid bottle capacity 2 L sheath fluid, 2 L waste, 250 mL cleaner, 250 mL decontamination

Signal Processing 24-bit datapath Computer Interface

USB 2.0